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Original article
Austral J. Vet. Sci.
Vol 52, 9-18 (2020)
DOI: 10.4067/S0719-81322020000100103

Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes

1 Colegio de Postgraduados, Campus Montecillo, Montecillo, Estado de México, México; Escuela Superior de Ciencias Agropecuarias, Universidad Autónoma de Campeche, Escárcega, Campeche, México.
2 Unidad Regional Universitaria Suroeste, Universidad Autónoma Chapingo, Tabasco, México.
3 Colegio de Postgraduados, Campus Montecillo, Montecillo, Estado de México, México.
4 Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Morelos, México.
5 Escuela Superior de Ciencias Agropecuarias, Universidad Autónoma de Campeche, Escárcega, Campeche, México.
6 División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Tabasco, México.
Keywords: eosinophils selection IgA plasma protein

Submitted: 2019-05-08

Accepted: 2019-10-22

Published: 2020-01-01

*Corresponding author:
robgardu@hotmail.com

How to Cite

Cruz-Tamayo, A., González-Garduño, R., Torres-Hernández, G., Becerril-Pérez, C. M., Hernández-Mendo, O., Ramírez-Bribiesca, J. E., López-Arellano, M. E., Vargas-Magaña, J. J., & Ojeda-Robertos, N. F. (2020). Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes. Austral Journal of Veterinary Sciences, 52(1), 9–18. https://doi.org/10.4067/S0719-81322020000100103

License

Copyright (c) 2020 Alvar Cruz-Tamayo, Roberto González-Garduño, Glafiro Torres-Hernández, Carlos M. Becerril-Pérez, Omar Hernández-Mendo, Jacinto Efrén Ramírez-Bribiesca, María E. López-Arellano, Juan J. Vargas-Magaña, Nadia F. Ojeda-Robertos

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The objective of this study was to compare two segregation methods to select resistant and susceptible female Pelibuey lambs infected naturally with gastrointestinal nematodes (GINs) in relation to their haematological and immunological response. For 6 months, faeces and blood samples were taken fortnightly from 40 grazing 5-month-old female lambs. The lambs were classified according to two methods using faecal egg count (FEC) as a phenotypical trait. In the first (reference) method (M3SE, n = 22), resistant (RES) lambs had FEC lower than the mean – 3 standard errors, the susceptible (SUS) lambs levels higher than + 3 standard errors
(n = 10) and the intermediate (INT) lambs (n = 8) were categorised by having FECs between the two values. The second method (QUM) divided the population, using quartiles, into resistant (RES; 25%), intermediate (INT; 50%), and susceptible (SUS; 25%) lambs. The agreement between both methods was estimated using the Kappa index. The packed cell volume (PCV), total plasma protein (TPP) and peripheral eosinophils (EOS) were determined for each group. Serum was used to evaluate the IgA levels. PCV and TPP values were higher (P<0.01) in the RES lambs (31.5 ± 3.4 and 6.16 ± 0.5 g/dL by QUM, respectively, and 31.5 ± 3.9 and 6.24 ± 0.49 g/dL by M3SE, respectively) than the SUS lambs (28.1 ± 4.7 and 5.94 ± 0.5 g/d, respectively, by both methods). The EOS and IgA values increased with age. M3SE and QUM were in moderate agreement (Kappa = 0.43). We concluded that the two segregation methods allowed for the identification of the same female SUS lambs, but a greater number of animals were categorised phenotypically as resistant using the M3SE method. PCV and TPP can help to identify phenotypically resistant animals.

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References

  1. Alba-Hurtado F, Muñoz-Guzmán MA. 2012. Immune responses associated with resistance to haemonchosis in sheep. BioMed Res Intern 2013, article ID 162158.
  2. Amarante AFTD, Susin I, Rocha RA, Silva MB, Mendes CQ, et al. 2009. Resistance of Santa Ines and crossbred ewes to naturally acquired gastrointestinal nematode infections. Vet Parasitol 165, 273-280.
  3. Balic A, Bowles VM, Meeusen EN. 2000. The immunobiology of gastrointestinal nematode infections in ruminants. Adv Parasitol 45, 181-241.
  4. Bentounsi B, Meradi S, Cabaret J. 2012. Towards finding effective indicators (diarrhoea and anaemia scores and weight gains) for the implementation of targeted selective treatment against the gastro-intestinal nematodes in lambs in a steppic environment. Vet Parasitol 187, 275-279.
  5. Beraldi D, Craig BH, Bishop SC, Hopkins J, Pemberton JM. 2008. Phenotypic analysis of host-parasite interactions in lambs infected with Teladorsagia circumcincta. Int J Parasitol 38, 1567-1577.
  6. Bishop S. 2012. A consideration of resistance and tolerance for ruminant nematode infections. Frontiers in Genetics 3, 168.
  7. Byers SR, Kramer JW. 2010. Normal hematology of sheep and goats. In: Weiss DJ, KJ Wardrop (eds). Schalm’s veterinary hematology. 6th ed. Wiley-Blackwell, Ames, USA, Pp 836-842.
  8. Cloete SWP, Olivier JJ, Du Toit E, Dreyer FH. 2007. Genetic analysis of faecal worm egg count in South African Merinos under natural challenge. South African J Animal Sci 37, 237-247.
  9. Cringoli G, Rinaldi L, Veneziano V, Capelli G, Scala A. 2004. The influence of flotation solution, sample dilution and the choice of the McMaster technique in estimating the faecal egg count of gastrointestinal strongyles and Dicrocoelium dentriticum in sheep. Vet Parasitol 123, 121-131.
  10. Dawkins HJS, Windon RG, Eagleson GK. 1989. Eosinophil responses in sheep selected for high and low responsiveness to Trichostrongylus colubriformis. Intern J Parasitol 19, 199-205.
  11. Emery DL, Hunt PW, Le Jambre LF. 2016. Haemonchus contortus: the then and now, and where to from here? Intern J Parasitol 46, 755-769.
  12. Fakae BB, Musongong GA, Chiejina SN, Behnke JM, Ngongeh LA, et al. 2004. Variability in the resistance of the Nigerian West African Dwarf goat to abbreviated escalating trickle and challenge infections with Haemonchus contortus. Vet Parasitol 122, 51-65.
  13. Gauly M, Erhardt G. 2001. Genetic resistance to gastrointestinal nematode parasites in Rhön sheep following natural infection. Vet Parasitol 102, 253-259.
  14. González-Garduño R, Torres-Acosta JFJ, Chay-Canul AJ. 2014. Susceptibility of hair sheep ewes to nematode parasitism during pregnancy and lactation in a selective anthelmintic treatment scheme under tropical conditions. Res Vet Sci 96, 487-492.
  15. González-Garduño R, López-Arellano ME, Conde-Felipe MM, Mendoza-de Gives P, Aguilar-Marcelino L, et al. 2017. Immune and haematological parameters of Blackbelly ewes infected with gastrointestinal nematodes. Rev Colomb Cienc Pecu 30, 219-230.
  16. Greer AW, Hamie JC. 2016. Relative maturity and the development of immunity to gastrointestinal nematodes in sheep: an overlooked paradigm? Parasite Immunol 38, 263-272.
  17. Landis JR, Koch GG. 1977. The measurement of observer agreement for categorical data. Biometrics, 33,159-174.
  18. Macpherson AJ, McCoy KD, Johansen FE, Brandtzaeg P. 2008. The immune geography of IgA induction and function. Mucosal Immunol 1, 11. Mavrot F, Hertzberg H, Torgerson P. 2015. Effect of gastro-intestinal nematode infection on sheep performance: a systematic review and meta-analysis. Parasites & Vectors 8, 557-568.
  19. McMahon C, McCoy M, Ellison SE, Barley JP, Edgar HWJ, et al. 2013. Anthelmintic resistance in Northern Ireland (III): Uptake of ‘SCOPS’ (Sustainable Control of Parasites in Sheep) recommendations by sheep farmers. Vet Parasitol 193, 179-184.
  20. McRae KM, McEwan JC, Dodds KG, Gemmell NJ. 2014. Signatures of selection in sheep bred for resistance or susceptibility to gastrointestinal nematodes. BMC genomics 15, 637.
  21. McRae KM, Stear MJ, Good B, Keane OM. 2015. The host immune response to gastrointestinal nematode infection in sheep. Parasite Immunol 37, 605-613.
  22. Morris CA, Vlassoff A, Bisset SA, Baker RL, Watson TG, et al. 2000. Continued selection of Romney sheep for resistance or susceptibility to nematode infection: estimates of direct and correlated responses. Animal Sci 70, 17-27.
  23. Morris CA, Wheeler M, Watson TG, Hosking BC, Leathwick DM. 2005. Direct and correlated responses to selection for high or low faecal nematode egg count in Perendale sheep. New Zealand J Agricul Res 48, 1-10.
  24. Morteo-Gómez R, González-Garduño R, Torres-Hernández G, NuncioOchoa G, Becerril-Pérez C, et al. 2004. Efecto de la variación fenotípica en la resistencia de corderos Pelibuey a la infestación con nematodos gastrointestinales. Agrociencia 38, 395-404.
  25. Palomo-Couoh JG, Aguilar-Caballero AJ, Torres-Acosta JF, Magaña-Monforte JG. 2016. Evaluation of different models to segregate Pelibuey and Katahdin ewes into resistant or susceptible to gastrointestinal nematodes. Trop Animal Health Prod 48, 1517-1524.
  26. Peña-Espinoza M, Thamsborg SM, Desrues O, Hansen TV, Enemark HL. 2016. Anthelmintic effects of forage chicory (Cichorium intybus) against gastrointestinal nematode parasites in experimentally infected cattle. Parasitology 143, 1279-1293.
  27. Preston SJM, Sandeman M, González J, Piedrafita D. 2014. Current status for gastrointestinal nematode diagnosis in small ruminants: where are we and where are we going? J Immunol Res 1-12.
  28. Rose H, Rinaldi L, Bosco A, Mavrot F, De Waal T, et al. 2015. Widespread anthelmintic resistance in European farmed ruminants: a systematic review. Vet Record 176, 546-546.
  29. SAS, Statistical Analysis System. 2004. SAS Version 9.2. SAS Institute Inc., Cary, NC, USA.
  30. Shaw RJ, Morris CA, Wheeler M. 2013. Genetic and phenotypic relationships between carbohydrate larval antigen (CarLA) IgA, parasite resistance and productivity in serial samples taken from lambs after weaning. Intern J Parasitol 43, 661-667.
  31. Smith WD, Jackson F, Jackson E, Williams J. 1985. Age immunity to Ostertagia circumcincta: comparison of the local immune responses of 4½ and 10-month-old lambs. J Comparative Pathol 95, 235-245.
  32. Stear MJ, Fitton L, Innocent GT, Murphy L, Rennie K, et al. 2007. The dynamic influence of genetic variation on the susceptibility of sheep to gastrointestinal nematode infection. J Roy Soc Interface 4, 767-776.
  33. Sweeney T, Hanrahan JP, Ryan MT, Good B. 2016. Immunogenomics of gastrointestinal nematode infection in ruminants-breeding for resistance to produce food sustainably and safely. Parasite Immunol 38, 569-586.
  34. van Wyk JA, Mayhew E. 2013. Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: a practical lab guide. The Onderstepoort J Vet Res 80, 539.
  35. Yacob HT, Mistre C, Adem AH, Basu AK. 2009. Parasitological and clinical responses of lambs experimentally infected with Haemonchus contortus (L3) with and without ivermectin treatment. Vet Parasitol 166, 119-123.
  36. Zaragoza-Vera CV, Aguilar-Caballero AJ, González-Garduño R, ArjonaJiménez G, Zaragoza-Vera M, et al. 2019. Variation in phenotypic resistance to gastrointestinal nematodes in hair sheep in the humid tropics of Mexico. Parasitol Res 118, 567-573.
  37. Zaros LG, Neves MRM, Benvenuti CL, Navarro AMC, Sider LH, et al. 2014. Response of resistant and susceptible Brazilian Somalis crossbreed sheep naturally infected by Haemonchus contortus. Parasitol Res 113, 1155-1161.

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