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Austral J. Vet. Sci.
Vol 56, 129-134 (2024)
DOI: 10.4206/ajvs.563.01

Serological evidence of Coxiella burnetii in sheep herds from Lonquimay valley in the Chilean Andes

1 Departamento de Ciencias Veterinarias y Salud Pública, Facultad de Recursos Naturales, Universidad Católica de Temuco, Chile.
2 Programa de Desarrollo Territorial Indígena (PDTI), Instituto Nacional de Desarrollo Agropecuario (INDAP), Chile.
Keywords: Q Fever Coxiellosis sheep prevalence zoonoses Chile

Submitted: 2024-02-08

Accepted: 2024-07-04

Published: 2024-08-22

*Corresponding author:
oalocilla@uct.cl

How to Cite

Alocilla-Velásquez, O., Navarrete, S. ., Alfaro-Chat, C. ., & Padilla, R. . (2024). Serological evidence of Coxiella burnetii in sheep herds from Lonquimay valley in the Chilean Andes. Austral Journal of Veterinary Sciences, 56(3), 129–134. https://doi.org/10.4206/ajvs.563.01

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Copyright (c) 2024 Oscar Alocilla-Velásquez, Scarlet Navarrete, Carmina Alfaro-Chat, Robert Padilla

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Coxiella burnetii is the causative agent of Q Fever, a worldwide zoonotic disease that causes acute flu-like illness and chronic manifestations in humans in the form of endocarditis, hepatitis, and other symptoms. Domestic ruminants are the most important reservoirs of the bacteria, transmitting infections to humans during the calving/lambing season through direct contact with contaminated fetal tissues or inhalation of dust particles. The aim of this study was to provide serological evidence and estimate the individual true seroprevalence of C. burnetii exposure in sheep herds in Lonquimay, and to characterize farmers’ knowledge of coxiellosis. A disease freedom survey was conducted in 30 sheep herds selected from the Indigenous Territorial Development Program database (PDTI). A total of 260 blood samples were tested using ELISA for C. burnetii antibody detection. Disease freedom and true animal-level prevalence were estimated, and a questionnaire was administered during farm visits to characterize farmers’ zoonotic knowledge. A positive result was found in 3% (1) of the sampled herds, and the true animal prevalence (mTALP) was higher than previous unpublished estimations (mTALP 4.2%, 95% PPI 1.6%–9%). The estimated probability of the study sheep population not being free of C. burnetii was 34%. A lack of knowledge regarding C. burnetii or the consequences of Q Fever was detected, along with risky behaviors that could facilitate pathogen transmission. This study revealed evidence of exposure to C. burnetii, with low individual and herd-level prevalence. Initiatives to improve zoonotic knowledge among farmers need to be implemented in the short term.

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References

  1. Amin, F., Ali, S., Javid, A., Imran, M., Rashid, M. I., Mertens-Scholz, K., & Neubauer, H. (2022). Sero-Epidemiology of Coxiella burnetii Infection in Small Ruminants in the Eastern Region of Punjab, Pakistan. Pathogens, 11(6). https://doi.org/10.3390/pathogens11060664
  2. Angelakis, E., & Raoult, D. (2010). Q fever. Veterinary Microbiology, 140(3), 297-309. https://doi.org/10.1016/j.vetmic.2009.07.016
  3. Baez, K. (2005). Impacto de la innovación tecnológica en la sustentabilidad de los sistemas de producción de campesinos pehuenches. Comuna de Lonquimay. IX Región de la Araucanía [Undergraduate Thesis, Universidad de Chile]. https://repositorio.uchile.cl/handle/2250/101788
  4. Bellini, C., Magouras, I., Chapuis-Taillard, C., Clerc, O., Masserey, E., Peduto, G., Peter, O., Schaerrer, S., Schuepbach, G., & Greub, G. (2014). Q fever outbreak in the terraced vineyards of Lavaux, Switzerland. New Microbes and New Infections, 2(4), 93-99. https://doi.org/10.1002/nmi2.37
  5. Berri, M., Souriau, A., Crosby, M., Crochet, D., Lechopier, P., & Rodolakis, A. (2001). Relationships between the shedding of Coxiella burnetii, clinical signs and serological responses of 34 sheep. Vet Record, 148(16), 502-505. https://doi.org/10.1136/vr.148.16.502
  6. Biggs, H. M., Turabelidze, G., Pratt, D., Todd, S. R., Jacobs-Slifka, K., Drexler, N. A., McCurdy, G., Lloyd, J., Evavold, C. L., Fitzpatrick, K. A., Priestley, R. A., Singleton, J., Sun, D., Tang, M., Kato, C., Kersh, G. J., & Anderson, A. (2016). Coxiella burnetii Infection in a Community Operating a Large-Scale Cow and Goat Dairy, Missouri, 2013. American Journal of Tropical Medicine and Hygiene, 94(3), 525-531. https://doi.org/10.4269/ajtmh.15-0726
  7. Cameron, A. R., & Baldock, F. C. (1998). A new probability formula for surveys to substantiate freedom from disease. Preventive Veterinary Medicine, 34(1), 1-17. https://doi.org/10.1016/S0167-5877(97)00081-0
  8. Carrié, P., Barry, S., Rousset, E., de Crémoux, R., Sala, C., Calavas, D., Perrin, J.-B., Bronner, A., Gasqui, P., Gilot-Fromont, E., Becker, C. A. M., Gache, K., & Jourdain, E. (2019). Swab cloths as a tool for revealing environmental contamination by Q fever in ruminant farms. Transboundary and Emerging Diseases, 66(3), 1202-1209. https://doi.org/10.1111/tbed.13137
  9. Catrileo, A., & Alvarado, C. (2009). GTT en Lonquimay: Ganadería de veranadas e invernadas en el sector Ranquil. Tierra Adentro, 86. Avalaible in https://hdl.handle.net/20.500.14001/5109. Last Accesed 30-12-2023.
  10. Cornejo, J., Araya, P., Ibáñez, D., Hormazabal, J., Retamal, P., Fresno Ramírez, M., & Lapierre, L. (2019). Identification of Coxiella burnetii in Tank Raw Cow Milk: First Findings from Chile. Vector-Borne and Zoonotic Diseases, 20(3). https://doi.org/10.1089/vbz.2019.2535
  11. Dijkstra, F., van der Hoek, W., Wijers, N., Schimmer, B., Rietveld, A., Wijkmans, C. J., Vellema, P., & Schneeberger, P. M. (2012). The 2007-2010 Q fever epidemic in the Netherlands: Characteristics of notified acute Q fever patients and the association with dairy goat farming. FEMS Immunology and Medical Microbiology, 64(1), 3-12. https://doi.org/10.1111/j.1574-695X.2011.00876.x
  12. Dodds, M. G., & Vicini, P. (2004). Assessing Convergence of Markov Chain Monte Carlo Simulations in Hierarchical Bayesian Models for Population Pharmacokinetics. Annals of Biomedical Engineering, 32(9), 1300-1313. https://doi.org/10.1114/B:ABME.0000039363.94089.08
  13. EFSA. (2010). Panel on Animal Health Welfare: Scientific Opinion on Q fever. EFSA Journal, 8(5), 1595. https://doi.org/10.2903/j.efsa.2010.1595
  14. Epelboin, L., De Souza Ribeiro Mioni, M., Couesnon, A., Saout, M., Guilloton, E., Omar, S., De Santi, V. P., Davoust, B., Marié, J. L., Lavergne, A., Donato, D., Guterres, A., Rabier, S., Destoop, J., Djossou, F., Baudrimont, X., Roch, A., Cicuttin, G. L., Rozental, T., Nacher, M., Millán, J., De Lemos, E. R. S., Fernandes, J., Duron, O., De Thoisy, B., & Rousset, E. (2023). Coxiella burnetii Infection in Livestock, Pets, Wildlife, and Ticks in Latin America and the Caribbean: a Comprehensive Review of the Literature. Current Tropical Medicine Reports, 10(3), 94-137. https://doi.org/10.1007/s40475-023-00288-7
  15. Guatteo, R., Seegers, H., Taurel, A.-F., Joly, A., & Beaudeau, F. (2011). Prevalence of Coxiella burnetii infection in domestic ruminants: A critical review. Veterinary Microbiology, 149(1), 1-16. https://doi.org/10.1016/j.vetmic.2010.10.007
  16. Hernández-Agudelo, J. M., Bartolotti, C., Tejeda, C., Tomckowiak, C., Soto, J. P., Steuer, P., Ulloa, F., & Salgado, M. (2023). Molecular and serological prevalence of Coxiella burnetii in bovine dairy herds in southern Chile: A PCR and ELISA-based assessment of bulk tank milk samples. Acta Tropica, 247, 107008. https://doi.org/10.1016/j.actatropica.2023.107008
  17. Lunn, D., Spiegelhalter, D., Thomas, A., & Best, N. (2009). The BUGS project: Evolution, critique and future directions. Statistics in Medicine, 28(25), 3049-3067. https://doi.org/10.1002/sim.3680
  18. Lurier, T., Rousset, E., Gasqui, P., Sala, C., Claustre, C., Abrial, D., Dufour, P., de Crémoux, R., Gache, K., Delignette-Muller, M. L., Ayral, F., & Jourdain, E. (2021). Evaluation using latent class models of the diagnostic performances of three ELISA tests commercialized for the serological diagnosis of Coxiella burnetii infection in domestic ruminants. Veterinary Research, 52(1), 56. https://doi.org/10.1186/s13567-021-00926-w
  19. Ministerio de Agricultura. (2000). Declara enfermedad infecto contagiosa y denuncia obligatoria y objeto de medidas sanitarias a las enfermedades de los animales denominada aborto zoonotico ovino y Fiebre Q de los ovinos. caprinos y bovinos. Avalaible in. https://www.sag.cl/content/declara-enfermedad-infecto-contagiosa-y-de-denuncia-obligatoria-y-objeto-de-medidas-sanitarias-las-enfermedades-de-los-animales-denominada-aborto-enzootico-ovino-y-fiebre-q-de-los-ovinos-caprinos-y-bovinos.
  20. Ministerio de Salud. (2017). Recomendaciones de manejo clínico y protocolo de estudio de laboratorio de brote de Fiebre Q.
  21. Palmer, N. C., Kierstead, M., Key, D. W., Williams, J. C., Peacock, M. G., & Vellend, H. (1983). Placentitis and Abortion in Goats and Sheep in Ontario Caused by Coxiella burnetii. The Canadian Veterinary Journal, 24(2), 60-61. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790252/pdf/canvetj00267-0042.pdf
  22. Rojas, C., Cáceres, L., & Tapia, B. (2022). Análisis de reultados del VIII Censo Nacional Agropecuario y Forestal. Oficina de Estudios y Politicas Agrarias, Ministerio de Agricultura Retrieved from https://www.ine.cl/estadisticas/economia/agricultura-agroindustria-y-pesca/censos-agropecuarios
  23. Rosenfeld, C. (2022). Progress in knowledge of the Q Fever in Chile. XIV Congreso Chileno de Buiatría,
  24. Santibañez, F., Santibañez, P., Caroca, C., Gonzalez, P., Perry, P., Gajardo, N., Sierra, H., Franchi, J., Huiza, F., Manriquez, B., & Muñoz, C. (2017). Atlas agroclimatico de Chile, Tomo IV, Regiones del Bíobío y de La Araucanía.
  25. Schneeberger, P. M., Wintenberger, C., van der Hoek, W., & Stahl, J. P. (2014). Q fever in the Netherlands – 2007–2010: What we learned from the largest outbreak ever. Médecine et Maladies Infectieuses, 44(8), 339-353. https://doi.org/10.1016/j.medmal.2014.02.006
  26. Sergeant, E. (2018). Epitools Epidemiological Calculators. Ausvet. Available at: http://epitools.ausvet.com.au
  27. Stevenson, M., Nunes, T., Heuer, C., Marshall, J., Sanchez, J., Thornton, R., Reiczigel, J., Robison-Cox, J., Sebastiani, P., Solymos, P., Yoshida, K., & Firestone, S. (2015). epiR: An R package for the analysis of epidemiological data. In (Version R package version 0.9-69.)
  28. Straily, A., Dahlgren, F. S., Peterson, A., & Paddock, C. D. (2017). Surveillance for Q Fever Endocarditis in the United States, 1999–2015. Clinical Infectious Diseases, 65(11), 1872-1877. https://doi.org/10.1093/cid/cix702
  29. Sturtz., S., Uwe Ligges, & Gelman, A. (2005). R2WinBUGS: A Package for Running WinBUGS from R. Journal of Statistical Software, 12(3), 16. https://doi.org/10.18637/jss.v012.i03
  30. Tapia, T., Olivares, M. F., Stenos, J., Iglesias, R., Díaz, N., Vergara, N., Sotomayor, V., Gallegos, D., Soares Magalhães, R. J., Acevedo, J., Araya, P., Graves, S. R., & Hormazabal, J. C. (2021). National Seroprevalence of Coxiella burnetii in Chile, 2016-2017. Pathogens, 10(5). https://doi.org/10.3390/pathogens10050531
  31. Tapia, T., Stenos, J., Flores, R., Duery, O., Iglesias, R., Olivares, M. F., Gallegos, D., Rosas, C., Wood, H., Acevedo, J., Araya, P., Graves, S., & Hormazabal, J. C. (2020). Evidence of Q Fever and Rickettsial Disease in Chile. Tropical medicine and infectious disease, 5(2), 99. https://doi.org/10.3390/tropicalmed5020099
  32. van Woerden, H. C., Mason, B. W., Nehaul, L. K., Smith, R., Salmon, R. L., Healy, B., Valappil, M., Westmoreland, D., de Martin, S., Evans, M. R., Lloyd, G., Hamilton-Kirkwood, M., & Williams, N. S. (2004). Q fever outbreak in industrial setting. Emerging Infectious Diseases, 10(7), 1282-1289. https://doi.org/10.3201/eid1007.030536
  33. Verdugo, C., Valdes, M. F., & Salgado, M. (2018). Within-herd prevalence and clinical incidence distributions of Mycobacterium avium subspecies paratuberculosis infection on dairy herds in Chile. Preventive Veterinary Medicine, 154, 113-118. https://doi.org/10.1016/j.prevetmed.2018.03.022
  34. Weitzel, T., López, J., Acosta-Jamett, G., Edouard, S., Parola, P., & Abarca, K. (2016). Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions. BMC Infectious Diseases, 16(1), 541. https://doi.org/10.1186/s12879-016-1880-9

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